【摘要】 利用细胞学和ＲＡＰＤ 技术，对从小麦与中间偃麦草杂种后代中选育的抗白粉病异附加系ＤＡＬ６６ 进行了鉴定。结果证明ＤＡＬ６６ 根尖细胞染色体数为４４，花粉母细胞减数第一分裂中期（ＰＭＣ ＭＩ）染色体构型为２ｎ＝ ２２Ⅱ。对ＤＡＬ６６ 及其双亲进行ＲＡＰＤ 分析，从４０ 个随机引物中筛选出１ 个特异引物（ＯＰＥ－０２）能够稳定地扩增出特异带型。更多还原

【Abstract】 Wheat- Elytrigia intermedium alien addition line (DAL66) with resistance to powdery mildew was identified by cytology and RAPD analysis.The results showed that chromosome number of DAL66 in root tip cell was 44,chromosome configuration of DAL66 in PMC MI was 2n=22Ⅱ.DAL66 and its parents were furtherly analyzed by RAPD method.40 random primers were used for RAPD amplification,the specific DNA band type can be amplified by one of them.更多还原