【摘要】 本文采用二茂铁作为电子传递介体 ,利用蘑菇组织含有丰富的酪氨酸酶 ,将蘑菇组织肉浆固定在二茂铁 (PVC膜 )修饰石墨电极上 ,用电流法测定L 酪氨酸浓度 .研究了电极的制备和最佳实验条件 ,其线性响应范围为 2 0× 1 0 -4 ～ 4 5× 1 0 -3 mol·L 1,响应时间小于 5min ,电极寿命至少30d ,可用于实际样品分析 .更多还原

【Abstract】 A new approach for assembling amperometric mushroom pulp tissue based membrane electrode for determination of L tyrosine analysis is proposed. Ferrocene is used as a mediator of electron transfer between tyrosinase in mushroom tissue and a graphite electrode. The optimal operation conditions are studied. The linear response range of the biosensor is 2 0×10 -4 to 4 5×10 -3 mol·L -1 with response time of less than 5 min and lifetime of at least 30 d. The biosensor can be applied to practical sample analysis.更多还原