【摘要】 目的 :探讨生殖道沙眼衣原体感染与输卵管性不孕的关系。方法 :用滤纸条取液技术获取输卵管液及宫颈粘液 ,通过多聚酶链反应检测沙眼衣原体的脱氧核糖核酸 ,60名输卵管性不孕患者作为观察者 ,因绝育或妇科良性及非感染性疾病手术输卵管外观正常的30名育龄期女性作为对照组。结果 :宫颈沙眼衣原体阳性率在两组间无明显不同 ,但输卵管沙眼衣原体阳性率(48 33 %)明显高于对照组(13 33 % ,P<0 01) ,其中输卵管不同病变间的阳性率也无明显差异。结论 :滤纸条取液技术取样作沙眼衣原体的多聚酶链反应检测效果好。输卵管不孕患者的输卵管沙眼衣原体感染率高 ,对输卵管性不孕患者应优先考虑沙眼衣原体感染更多还原

【Abstract】 Objective: To probe into the relationship between Chlamydia trachomatis (CT) infection and tubal factor infertlility (TFI). Methods: Using filter paper technique to obtain samples from tubes and cervixes and then to detect CT-DNA by polymerase chain reaction (PCR) in both test group and control group. 60 TFI female patients were chosen as the test group while 30 reproductive age women, who underwent tubal ligation or uterus surgery due to benign and noninfectious diseases and whose tubes are morphologically normal, as the control group. Results: The positive rates of cervical CT infection in both the test group (TFI group) and the control group are not significantly different.The positive rate of tubal CT infection in the test group (48.33%), however,is much higher than that in the test group (13.33%,P<0.01).The positive rates between different types of tubal pathological changes are not obviously different.Conclusions: The filter paper technique is an effective method in detecting CT-DNA by PCR. TFI women often show a high CT infection rate and therefore TFI patients should be firstly suspected if they have suffered from TC infection in the clinical practice.更多还原