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两株小鼠肝癌细胞增殖状态比较研究

The comparison between two hepatic tumor cell lines with different metastatic capacity on cell distribution

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【作者】 张晨董瑞琪侯力白洁凌茂英

【Author】 ZHANG Chen,DONG Rui-qi,HOU Li (Hematology Institute of Traditional Chinese Medicine,The 210th Hospital,Dalian 116021 China)

【机构】 解放军第210医院中医血液病研究所!辽宁大连116021大连陆军学院!辽宁大连116100大连医科大学病理学教研室!辽宁大连116027

【摘要】 目的 :探讨细胞增殖状态与细胞转移力的关系。方法 :采用流式细胞术 ,测定两株腹水型肝癌细胞的DNA含量 ,进而提供细胞增殖状态的信息。结果 :F和P细胞的DNA指数及细胞增殖指数均无显著性差异。F细胞的淋巴结转移瘤的G2 /M期比率高于其原发瘤。结论 :两株细胞的转移率与细胞的增殖状态无明显的相关性

【Abstract】 Objective:To investigate the relationship of cell cycle distribution and metastasis in two tumor cell lines.Methods:The DNA contents of two hepatic carcinoma cell lines were studied by flow cytometry.Results:The DNA index and proliferative index between higher metastatic F cell and lower metastatic P cell were no significant differences,but G 2/M phase of tumor cells with lymphatic metastasis were remarkably higher than that of F cell in non-metastasis.Conclusion:The two hepatic carcinoma cell lines with different lymph node metastatic capacities were no differences in cell proliferation.

【关键词】 流式细胞术细胞株细胞周期
【Key words】 flow cytometrycell Linescell cycle distribution
  • 【文献出处】 大连医科大学学报 ,JOURNAL OF DALIAN MEDICAL UNIVERSITY , 编辑部邮箱 ,2000年03期
  • 【分类号】R735.7
  • 【被引频次】1
  • 【下载频次】74
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