【摘要】 目的 :进一步了解脐血造血细胞回巢潜能及硫酸葡聚糖 (DS)的动员作用机制。 方法 :重症联合免疫缺陷小鼠经亚致死剂量γ射线照射或 DS预处理后 ,进行经冻存后的人脐血移植 ,利用人粒 -单系祖细胞培养及流式细胞术 ,检测植入小鼠骨髓、脾脏等器官中的人源性细胞。 结果 :与未预处理组相比 ,DS或照射预处理组移植受体鼠内人源 CD45 + ,CD34+ 及CD19+细胞植入水平明显提高 ;DS预处理组植入水平虽较照射预处理组差 ,但毒性较低 ,小鼠存活率高达 10 0 %。 结论 :DS是一种安全、有效的预处理方案 ;DS对造血干细胞既有动员作用又有促进龛位腾出以供移植干细胞回巢的作用。更多还原

【Abstract】 Objective: To better understand homing potentiality of hematopoietic stem cells (HSC) in human umbilial cord blood (UCB) and the mechanism of dextran sulfate (DS) mobilization. Methods: Sub lethally irradiated or DS pretreated severe combined immunodeficient (SCID) mice were transplanted with UCB, which was cryopreserved at -80℃.Human cells in recipient mice were detected by flow cytometry and CFU GM assay from each host organ. Results: In contrast with the controls, engraftment after irradiation or administration of DS resulted in a higher percentage of CD45 +,CD34 +,CD19 + cells produced in SCID mice. While comparison between the experimental groups, higher implantation level was obtained in the former if equivalent donor cells were used in both groups. Conclusion:DS is a safe and effective pretreatment, which can mobilize HSC, but also vocate niches for transplanted HSC homing. [更多还原