【摘要】 目的研究缺氧缺血性脑损伤（ＨＩＢＤ）后血和脑脊液中Ｓ－１００蛋白（Ｓ—１００）、神经元特异性烯醇化酶（ＮＳＥ）水平的变化及其与脑细胞死亡数的相关性，并探讨这些蛋白质水平变化的机制。方法采用 ７ ｄ龄 ＳＤ大鼠ＨＩＢＤ模型，应用放射免疫方法动态观察ＨＩＢＤ血液和脑脊液中Ｓ—１００，ＮＳＥ水平的变化，用ＲＴ－ＰＣＲ的技术和免疫组织化学的方法动态观察 ＨＩＢＤ后不同时间点脑组织中 Ｓ— １００，ＮＳＥ ｍＲＮＡ和蛋白水平表达的变化。结果 ＨＩ后血液中 ２４ ｈ，４８ ｈ Ｓ－ １００分别为（１． ２０５± ０ １８３）μｇ／Ｌ和（ １． ２３５± ０．０９７）μｇ／Ｌ， ＮＳＥ分别为（３．９７ ±０．２２８）ηｇ／ｍｌ和（３．７６±０．２３４）ηｇ／ｍｌ，对照组Ｓ－１００和ＮＳＥ分别为（０．６４５±０．０５）μｇ／Ｌ和（３．１５±０．１６４）ηｇ／ｍｌ。脑脊液中 ２４ ｈ，４８ ｈ Ｓ－ １００分别为（１． ２８± ０． ０３１）μｇ／Ｌ，（１． ３２± ０． ０９７）μｇ／Ｌ，ＮＳＥ分别为（７． １５± ０． ７１７）ηｇ／ｍｌ，（４． ２９± ０．１４４）ηｇ／ｍｌ，对照组 Ｓ－ １００和 ＮＳＥ分别为（０． ６８± ０．０５９） μｇ／Ｌ和（３． ４?更多还原

【Abstract】 Objective To evaluate the relationship between changes of cerebrospinal fluid (CSF) or blood S - 100 protein (S- 100), neuron specific enolase (NSE) levels and the severity of hypoxic - ischernic encephalopathy (HIE), and to explore the mechanism of changes of these protein levels. Method Seven - day postnatal SD rats were used. Their serial blood and CSF S - 100 and NSE were measured by radioimmunoassay. By using the RT - PCR technique the expression of mRNA for S - 100 and NSE in the brain tissue at different gluts of time after HI injury was tested. Immunohistochemical assay was used to investigate the changes of the expression of S - 100 and NSE at the protein level. Results The values of S - 100, NSE were (1. 205 ± 0. 183) μg/L and (3. 97 ± 0. 228) ηg/ml respectively at 24 hours and (1. 235 ± 0. 097) μg/L, (3. 76 ± 0. 234) ηg/ml respectively at 48 hours in the blood, were(1. 28 ± 0. 031 ) μg/L, (7. 15 ± 0. 717) ηg/ml respectively at 24 hours and (1. 32 ± 0. 097) μg/L, (4. 29 ± 0. 144)ηg/ml respectivaly at 48 hours in the CSF after HI injury. The values of S - 100, NSE were increased significantly in both the blood and CSF samples during 24-48 hrs after HI inujry than in the control group (0. 645 ± 0. 05 μg/L, 3. 15 ± 0. 164 ηg/ml and 0. 68 ± 0. 059 μg/L, 3. 42 ± 0. 322 ηg/ml respectively). The increment corresponded well with death cell counts in the brain after HI injury; a peak of rnRNA expression of NSE exnerged at 24 hours after HI injury, while S- 100 mRNA occurred at 48 hours. The positive area of the expression of S - 100 at the protein level increased progressively during 12-96 hrs, but the expression of NSE decreased significantly with the lapse of time. Conclusions S - 100 and NSE are senstitive markers for hypoxic-ischemic brain damage(HIBD). The appropriate time for sample collection is 24 - 48 hrs after HI injury. The mechanism of S - 100 level increase in the blood and CSF after HI injury is not only due to the leakage from damaged brain cells but also through a high expression Of S - 100 mRNA and S - 100 protein, while the elevation of NSE in the blood and CSF was mainly due to the leakage from neuronal damage.更多还原