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外源脯氨酸对盐胁迫下大豆离体胚再生植株生理特征及线粒体结构的影响
EFFECTS OF EXOGENOUS PROLINE ON THE PHYSIOLOGICAL PROPERTIES AND THE MITOCHONDRIA ULTRASTRUCTURE IN SOYBEAN REGENERATED PLANTLETS FROM EMBRYOS IN VITRO UNDER NaCl STRESS
【摘要】 以“晋豆八号”作材料,利用组织培养方法研究盐胁迫下外源脯氨酸对大豆离 体胚再生植株的膜脂过氧化水平、保护酶系统、可溶性蛋白质及线粒体超微结构的影 响。结果表明:在外源脯氨酸的作用下,大豆再生植株体内 MDA含量减少,膜相对透 性降低,SOD、POD两种酶活性明显提高,可溶性蛋白质含量增加;电镜观测显示,外 源脯氨酸可以有效缓解盐胁迫对大豆离休胚再生植株线粒体结构的损伤。
【Abstract】 The salt resistance effect of exogenous proline in soybean regenerated plantlets from embryos in vitro were studied by means of tissue culture. The results showed that exogenous proline could reduce the malondialdehyde (MDA) content, product of lipid peroxidation, and the relative permeability of plasma membrane in the soybean plantlets under NaCI stress. The activities of POD and SOD increased, the soluble protein content also was promoted. Exogenous proline alleviated the injury of the mitochondria ultrastructure of the soybean regenerated plantlets.
【Key words】 Exogenous proline; Soybean; Mitochondria; Lipid peroxidation;
- 【文献出处】 大豆科学 ,Soybean Science , 编辑部邮箱 ,2000年04期
- 【分类号】S565.1
- 【被引频次】42
- 【下载频次】245