【摘要】 piggyBac转座子在分类上属于真核生物的第二类转座子 ,是一个自主因子 ,长 2 4 76bp ,有短的末端反向重复序列 (ITR)和一个可读编码框 (ORF) ,ITR长 1 3bp ,ORF长 2 1kb。piggyBac转座子可以在基因组中切出和转座 ,切出和转座总是发生在特征性的TTAA四核苷酸目标位点序列 ,转座频率较高 ,而且piggyBac转座子受生物体种类的限制性较少。利用piggyBac转座子构成的载体———辅助质粒系统已成功地获得了转基因地中海果蝇、黑腹果蝇和家蚕。更多还原

【Abstract】 piggyBac transposon, whose size is 2 5 kb,with 13bp inverted terminal repeats(ITR) and a 2 1kb open reading frame(ORF),is claasified as type Ⅱ transpososn element of eucaryote.Exciesion and transposion of piggyBac transposon occurs among genomes with higher frequency in characteristic tetranucleotide TTAA target site sequence.piggyBac transposon is less restricted by the species of organsim.Transgenic Mediterranean fruit fly, Drosophila melanogaster , and silkworm, Bombyx mori , have been successfully obtained with vector helper plasmid system which is constituted by piggyBac transposon.更多还原