【摘要】 对碱裂解法进行了改良，采用不同ｐＨ值的均浆缓冲液从家蚕５龄幼虫后部丝腺、蛹和卵中提取ｍｔＤＮＡ，调查其得率和纯度，建立了家蚕线粒体ＤＮＡ的经济快速提取方法。更多还原

【Abstract】 Adopting a modified alkaline lysis procedure, mitochondrial DNA (mtDNA) was isolated from the posterior silkgland of 5th instar larvae of silkworm (Bombyx mori)and its pupae and eggs in homogeniz- ing buffers with different PH values. The recovery rate of mtDNA and its purity was recorded, thus suc- cessfully constructing a new economical and rapid procedure for the isolation of mtDNA of B. mori.更多还原