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几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果
Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro
【摘要】 构建了几丁质酶基因植物表达载体 ,该基因在大肠杆菌中能表达 35k D的蛋白质。几丁质酶粗提物在体外对柱花草胶孢炭疽病原菌有极显著的抑菌效果
【Abstract】 Chinase gene expressing vectors for plant transformation were constructed,35kD protein was expressed by chitinase gene in Escherichiacoli DH5α. In vitro crude preparation of chinase has efficient inhibition on the growth of Colletotrichum gloeosporioides, which caused Stylosnathes anthracnose disease.
【关键词】 几丁质酶;
基因表达;
炭疽病原菌;
抑菌作用;
【Key words】 Chinase gene; Expressing in Escherichia coli; Inhibition on Colletotrichum gloeosporioides growth;
【Key words】 Chinase gene; Expressing in Escherichia coli; Inhibition on Colletotrichum gloeosporioides growth;
【基金】 国家外国专家局和国际热带农业中心资助
- 【文献出处】 草地学报 ,ACTA AGRESTIA SINICA , 编辑部邮箱 ,2000年02期
- 【分类号】S432
- 【被引频次】20
- 【下载频次】160