【摘要】 目的 :观察人类内源性逆转录病毒长末端重复序列 (humanretroviruslongterminalrepeat,HRTVLTR)在转移和非转移性肺癌中的表达 ,探讨HRTVLTR激活与肺癌转移的关系。方法 :采用PCR扩增 17例肺癌病人原发灶、邻近淋巴结及癌旁正常组织基因组中的内源性HRTVLTR ,证明内源性HRTVLTR的存在 ;以RNA斑点杂交检测 17例肺癌病人原发灶、邻近淋巴结及癌旁正常组织内源性HRTVLTR的表达 ,比较其在转移和非转移性肺癌中的表达差异。结果 :PCR结果显示 ,在转移能力不同的人肺腺癌细胞系 (AGZY 83a和Anip 973)及肺癌病人原发灶、邻近淋巴结及癌旁正常组织均获得了HRTVLTR扩增产物。 17例肺癌标本RNA斑点杂交分析发现 ,8例转移性非小细胞肺癌病人中有 5例原发灶及其转移淋巴结表现为HRTVLTR的高表达 ,4例小细胞肺癌无HRTVLTR的表达。结论 :人类基因中普遍存在内源性HRTVLTR ;HRTVLTR的高表达可能与非小细胞肺癌的转移有关更多还原

【Abstract】 Objective: To explore the relationship between the expression of the human retrovirus long terminal repeat (HRTV LTR) and the metastasis of human lung cancer. Methods: PCR was used to amplify the fragment in the primary tumors, metastatic lymph nodes and normal tissues of seventeen patients and RNA Dot Blot was used to detect its expression. Results: PCR results indicated that the fragment existed in all the above tissues. RNA Dot Blot revealed that five of eight metastatic non small cell lung cancer patients manifested higher expression in the metastatic lymph nodes than the primary tumors while no expression was detected in the four small cell lung cancer patients. Conclusion: The result suggested that the fragment existed in the genome of all the patients and it was the expression, not the insertion of the fragment that was responsible for the metastasis of the non small cell lung cancer.更多还原