【摘要】 目的 :研究雷公藤多甙 (tripterygiumwilfordiihookfmulti glycosides ,TWG)对角朊细胞株 (COLO 16 )的增殖抑制及对凋亡的影响 ,并探讨其治疗免疫性皮肤病的机制。方法 :MTT法检测细胞增殖抑制 ,二苯胺及PI染色法检测细胞凋亡 ,RT PCR测细胞bcl 2及bcl XsmRNA表达。结果 :TWG 0 .2 5mg·L-1与角朊细胞共育 72h后 ,角朊细胞增殖活性受抑 ,此抑制作用具药物 (质量 )浓度依赖性 ;TWG 1mg·L-1作用于角朊细胞 72h ,即可使片段化DNA含量及亚二倍体细胞百分数较对照明显增加 ,此现象随药物质量浓度增加而增高 ;TWG 5mg·L-1,可上调角朊细胞bcl XsmRNA表达 ,此作用随药物作用时间延长而增强 ;TWG对bcl 2mRNA表达则无明显影响。结论 :TWG可抑制角朊细胞株增殖并诱导其凋亡 ,此诱导凋亡机制可能与bcl Xs上调有关更多还原

【Abstract】 Objective: To investigate the effect of tripterygium wilfordii hook f multi glycosides(TWG) on proliferation and apoptosis of COLO 16 cells. Methods: Cell proliferation was measured using the MTT method; apoptosis was determined by DNA fragmentation and PI staining for Flow Cytometry assay; RT PCR was used to evaluate the expression of bcl Xs and bcl 2 mRNA. Results: Treatment of COLO 16 cells with TWG resulted in (1) significant inhibition of proliferation in a dose dependent manner, (2) The DNA fragmentation and hypodiploid cell formation, (3) Up regulation of bcl Xs mRNA.Conclusion: TWG induced apoptosis and inhibited the proliferation of COLO 16 cells. TWG might achieve this effect by up regulation the bcl X genes.更多还原