【摘要】 报道了三尖杉酯碱 (harringtonine ,HT)可以诱导HeLa细胞凋亡 .采用Heochst33342荧光染色、琼脂糖凝胶电泳及流式细胞光度术 (FCM)的方法 ,研究了HT对HeLa细胞凋亡的影响 .利用细胞同步化技术和斑点杂交法研究发现 ,HT影响了HeLa细胞c myc和bcl 2基因的表达并且与细胞周期密切相关 .初步探讨其凋亡诱导的机制 ,认为HT通过在G1和G2 期下调细胞凋亡抑制基因bcl 2的诱导凋亡 ,以及下调c myc癌基因阻滞细胞增殖 ,并延迟凋亡发生 .这些结果对于了解HT的药物作用机制和提高临床化疗疗效具有重要意义 .更多还原

【Abstract】 The anticancer drug harringtonine(HT) which can induce apopt os is is investigated in HeLa cells by Heochst33342 nucleic staining, agarose gel e lectrophoresis of DNA and flow cytometry. Typical apoptotic cell death is observ ed from 2 to 24 h and it increases in both dose-and time-dependent fashion. The mechanism of HT inducing HeLa apoptosis is studied with synchonized cells a nd measuring gene expression. The results demonstrate that the expression of bcl -2 decreases in G 1 and G 2 and keeps relatively stable in S phase, t his agree with FCM results that HT induces HeLa cell apoptosis and S phase cells increasing. In addition, HT induces down regulation of c-myc to inhibit cell p roliferation and delays the progression of apoptosis. These results are importan t to understand the mechanism of action of HT and to improve the effect of HT in cancer chemotherapy.更多还原