【摘要】 研制了含有nm级Ag颗粒的葡萄糖氧化酶电极 .结果表明 ,该电极具有很大的响应电流 ,在相同的条件下 ,其响应电流比文献所报导的含有Au或SiO2 的酶电极响应电流大 .当颗粒粒径为 4 .3～ 7.8nm时 ,酶电极响应电流随粒径的增大而增大 ;而当粒径继续增大时 ,其响应电流则随粒径的增大而减小 .对Ag颗粒在酶电极中的作用机理也进行了初步探讨 .更多还原

【Abstract】 An amperometric enzyme electrode based on immobilization of glucose oxidase(GOD) within silver(Ag) nanoparticles is presented. The enzyme electrodes are platinum electrodes covered with a membrane containing GOD and Ag nanoparticles. The results show that this kind of enzyme electrodes possess higher sensitivity than those containing Au or SiO 2 nanoparticles. Comparing the response current of these Ag GOD electrodes, it is found that the sensitivity of the enzyme electrodes increases with increasing Ag size in the case of 4.3 7.8 nm. In the case of Ag size increasing continously, the situation is different, the enzyme electrodes have lower sensitivity with increasing Ag size. The mechanism of this phenomenon is studied and discussed on the basis of the ability of Ag nanoparticles transfer electron and the adsorption of GOD onto ultrafine particles.更多还原