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文冠果变异株和野生型植株基因组差示杂交研究初报
Cloning the differences between the wild and mutant genomes of Xanthoceras sorbifolia Bunge.
【摘要】 文冠果 (XanthoccrassorbifoliaBunge)同源异型变异株是自然产生的花器官发生变异的个体 .应用扣除杂交技术对该变异株和野生型植株进行了基因组差示杂交 ,获得样本特异DNA片段 ,此DNA片段可以作为进一步筛选突变基因的探针 ,为克隆文冠果花发育调控基因奠定基础
【Abstract】 A natural flower homeotic mutant of Xanthoceras sorbifolia Bunge was studied by genome DNA subtraction between the mutant and wild plants. Through the subtraction, a wild plant specific genome DNA fragment has been found and this fragment will be used as a probe in following mutant gene screening study.
【关键词】 文冠果;
基因组差示杂交;
特异DNA片段;
【Key words】 Xanthoccras sorbifolia Bunge; genome DNA subtraction; specific DNA fragment;
【Key words】 Xanthoccras sorbifolia Bunge; genome DNA subtraction; specific DNA fragment;
【基金】 国家自然科学基金资助项目 !( 3 96 70 6 19)
- 【文献出处】 北京林业大学学报 ,JOURNAL OF BEIJING FORESTRY UNIVERSITY , 编辑部邮箱 ,2000年01期
- 【分类号】Q943
- 【被引频次】35
- 【下载频次】244