节点文献
含有重组人白细胞介素18基因的工程菌的产物表达和鉴定
Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin 18(IL-18) Gene
【Abstract】 The cDNA of human Interleukin\|18 was obtained by RT\|PCR from the mRNA of human peripheral blood cells.The DNA sequencing was performed and the DNA was cloned into expression vector pBV220.The overexpression was performed in E.coli HB101.The expressive product was purified through Sephadex G100 and identified by N\|terminal amino acid sequencing and Western blot.
- 【文献出处】 北京大学学报(自然科学版) ,ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS , 编辑部邮箱 ,2000年02期
- 【分类号】Q784
- 【被引频次】1
- 【下载频次】90