【摘要】 为了解甲型肝炎 (甲肝 )病毒 (HAV)在中国几个城市的基因型分布 ,选择浙江杭州、江苏启东、安徽铜陵、云南昆明和上海市等的甲肝病人粪便标本或血清标本 ,以逆转录 -套式聚合酶链反应 (RT nPCR)扩增合成HAVVP1/2A交接区基因区 ,并进行直接核苷酸序列分析和差异比较。结果表明 ,从这些城市甲肝病人分离到的 17株HAV株均属基因Ⅰ型 ,为IA和IB亚型 ;所有HAV株间核苷酸差异均小于 15 % ,但约 5 0 %HAV株株间核苷酸差异大于 7 5 % ,说明中国流行或散发的HAV株可能有基因IA、IB亚型同时存在。该项研究结果将为HAV的分子流行病学追踪调查提供方法和依据。更多还原

【Abstract】 In order to clarify the distribution of hepatitis A virus (HAV) genotypes in China, 17 HAV strains were isolated from fecal and serum specimens of hepatitis A patients in different geographical regions including Hangzhou Zhejiang, Qidong Jiangsu, Tongling Anhui, Kunming Yunnan and Shanghai. The nucleotide sequence of VP1/2A region of the 17 HAV isolates were tested by RT nPCR and direct sequencing. The nucleotide sequence diversity among all 17 HAV strains were less than 15%, but that in half of the isolates were greater than 7.5%. The results showed that epidemic or sporadic HAV strains isolated in China may belong to HAV genotype I, subgenotypes IA and IB.更多还原