【摘要】 1目的　检测非霍奇金病的微小残留病。 2方法　应用 PCR扩增琼脂糖凝胶电泳检测法 ,测定 5 0例非霍奇金病 bcl- 2基因表达与 t(14;18)染色体易位的关系。 3结果　在这个易位中 ,位于 18q2 1 上 bcl- 2原癌基因与 14q32 上的 Ig H基因融合 ,使 bcl- 2基因表达失常。本组 5 0例淋巴瘤病人中 ,11例 (2 2 % )表达 t(14;18)易位 ,5例经过治疗已获得缓解的病人中 ,1例检测到 t(14;18)易位 ,随访 90 d后临床复发。 4结论　 t(14;18)易位可作为人类细胞转化或肿瘤进展的重要分子标志之一 ,对于检测微小残留病有一定临床应用价值。更多还原

【Abstract】 Objective To detect minimal residual disease of non Hodykin lymphoma.MethodsThe relationship between the expression of bcl 2 gene and the chromosome translocation of t(14;18) was measured by polymerase chain reaction(PCR).Results In the translocation, the juxtaposition of the proto oncogene bcl 2 at chromosome segment 18q 21 and the Ig heavy chain locus at 14q 32 resulted in irregular expression of bcl 2. 50 patients with lymphomas were studied by polymerase chain reaction. 11 cases showed t(14;18) translocation (22%). t(14;18) translocation was detected in 1 of 5 cases in catabasis after treatment and this patient relapsed after three months.Conclusiont(14;18) translocation may be a remarkable molecular marker for transition of cells or progress of lymphomas and has a very important value for detecting minimal residual disease(MRD). [更多还原