【摘要】 目的 :探讨绞股蓝总甙对阿霉素致膈肌毒性的拮抗作用。方法 :家兔随机分为对照组、阿霉素组和绞股蓝总甙 +阿霉素组 ,分别注射生理盐水、阿霉素 (10mg/kg)和绞股蓝总甙 (10 0mg/kg) +阿霉素 (10mg/kg) ,2 4h后测膈肌诱发电位 (DEP)及跨膈压 (Pdi) ,电镜观察膈肌超微结构的变化 ,数据经方差分析和q检验。结果 :(1)与对照组相比 ,阿霉素能显著降低膈肌诱发电位幅度和跨膈压 (P <0 .0 1和P <0 .0 5 ) ;(2 )阿霉素可致膈肌细胞损伤 ,表现为肌小节Z线排列不整齐、紊乱 ,部分Z线模糊、消失 ,线粒体嵴呈管状 ,空泡变性 ,见板层状结构 ;(3)绞股蓝总甙可拮抗上述变化。结论 :绞股蓝总甙对阿霉素致膈肌毒性有拮抗作用。更多还原

【Abstract】 Objective:To study the antagonistic effect of gypenoside(GP) on diaphragm against adriamycin cytotoxicity in rabbits.Methods:The toxicant effect on diaphragm of doxorubicin and the antagonistic effect of GP were examined by measurements of diaphragm evoked potential(DEP) and the transdiaphragmatical pressure(Pdi) and observation of the ultrastructure of diaphragm.All data were evaluated by q test.Results:(1)The amplitude of DEP and the Pdi were markly decreased in rabbits treated with doxorubicin( P <0.01 and P < 0.05) ;(2)The disorder of myofibrills and swelling of mitochondria with broken cristae were observed in doxorubicin group;(3)The above mentioned changes were inhibited by gypenoside.Conclusions:The results showed that the gypenoside could antagonize the toxic effect of doxorubicin and protect the diaphragm myocytes. [更多还原