【摘要】 目的：探讨石斛多糖（ dendrobium candidum polysaccharide,DCP）与 rIL- 2联合应用对脐带血 LAK细胞（ CB-LAK）和肿瘤病人外周血 LAK细胞（ PB-LAK）体外杀伤肿瘤细胞作用的影响。方法：应用 3H-TdR释放法测定 CB-LAK和 PB-LAK细胞的杀伤活性。结果：① rIL-2(500 u/ml)能明显提高 CB-LAK对 Raji细胞的杀伤活性 ,0 h的 CB-LAK活性为 13.63％ ,rIL-2(500 u/ml)诱导 48、 72、 96、 120 h后 ,CB-LAK活性分别提高至 22.28％、 26.23％、 28.55％和 25.76％ (P0.05）；③ DCP（ 200 mg/L）与 rIL-2（ 500 u/ml）联合培养 96 h，显著增强 PB-LAK杀伤活性。 10例恶性肿瘤病人外周血（ DCP＋ rIL-2）组与单纯 rIL-2组诱导的 PB-LAK平均活性分别为 34.78％和 23.24％ ,P更多还原

【Abstract】 Objective: The current study was designed to explore the effect of combinative use of dendrobium candidum polysaccharide (DCP) and rIL 2 on killing activity of lymphokine activated killer cell (LAK) of umbilical cord blood (CB LAK) and peripharal blood of cancer patients (PB LAK) in vitro. Methods: The killing activity of CB LAK and PB LAK against Raji cells was determined by (3H) TdR release assay. Results: (1) The killing activity of CB LAK against tumor cells was markedly enhanced by IL 2 (500 u/ml). The killing rate of CB LAK was 13.63％ in at 0 h and was increased to 22.28％ , 22.63％ ,28.54％ and 25.76％ after rIL 2 inducing for 48 h,72 h,96 h and 120 h respectively (P inducing for 96 h was 34.78％ . But rIL 2 (500 u/ml) alone was only 23.24％ (P更多还原