【摘要】 目的： 研究 p53基因 249密码子在肝癌非高发区安徽省的点突变的发生率。方法：应用 PCR- RFLP技术分析肝细胞癌 p53基因 249密码子突变的频率。结果：所检测的 38例肝细胞癌均无出现 p53基因第七外显子纯合性缺失， 4例有 p53基因 249密码子的点突变，且均为杂合型突变，突变率为 10.53％ (4/38)。结论：本资料说明，在肝细胞癌非高发区中 ,肝细胞癌中 p53基因第七外显子发生的点突变并非为高发事件。提示 p53基因第七外显子的点突变在肝癌非高发区肝细胞癌的形成过程中可能不起主导作用。更多还原

【Abstract】 Objective： The current study was designed to investigate the point mutation of p53 gene codon 249 in hepatocellular carcinoma(HCC) of non- prevalent areas in Auhui, China. Methods： The PCR- RFLP was applied to analyze the rate of point mutation of p53 gene codon 249. Results： In this 38 cases of hepatocellular carcinoma, no homozygous deletion of p53 exon7 was detected and point mutation of codon 249 was found in 4 cases with a mutation rate of 10.53％ (4/38). Conclusion： Our data suggest that in the non- prevalent areas in Anhui, China, the incidence of point mutation of p53 codon 249 is not high in hepatocellular carcinoma, and the point mutation of p53 exon7 may not play a major role in the carcinogenesis of hepatoma.更多还原