【摘要】 目的 :检测基质金属蛋白酶（ matrix metalloproteinase 9,MMP－ 9）在各类人胃粘膜中的表达频率，并观察其分布特点及其与胃癌发生和发展的关系。材料和方法：采用免疫组织化学染色，组织选择性蛋白提取法 /Western印迹杂交和酶谱分析等方法，检测 27例非癌胃粘膜， 52例各类癌前病变和 65例胃癌标本以及 2株胃癌细胞系中 MMP 9的表达情况。 结果： MMP 9的检出率在非癌粘膜为 3.7％ (1/27)，萎缩性胃炎 69.2％ (9/13） ,肠上皮化生 70.0％ (7/10),Ⅱ型和Ⅲ型非典型增生分别是 86.7％ (13/15)和 78.6％ (11/14)；它在高、中、低和未分化胃癌中的表达率分别达 64.2％ (9/14）、 78.6％ (11/14)、 83.3％ (20/24)和 76.9％ (10/13)。 MMP 9在两株细胞系以及肿瘤间质纤维细胞亦呈阳性。结论 :MMP 9表达出现在胃癌发病的早期阶段；其表达率与胃癌进展正相关（ P<0.01)更多还原

【Abstract】 Objective: To investigate the expression and distribution of matrix metalloproteinase 9(MMP 9) in gastric mucosa with different morphology and to elucidate its association with tumor progression. Methods: Frozen tissues from 27 cases of none cancerous mucosa, 52 cases of variant pre neoplastic lesions, 65 cases of gastric cancers as well as two established gastric cancer cell lines were examined for expression of MMP 9 by immunocytochemical staining, Western blot combined with tissue selective protein extraction and zymography. Results: The positive rates of MMP 9 were 3.7％ (1/27) in none cancerous mucosa, 69.2％ (9/13) in atrophic gastritis, 70.0％ (7/10) in intestinal metaplasia,86.7％ (13/15) in GradeⅡ dysplasia,78.6％ (11/15) in GradeⅢ dysplasia,and 64.2％ (9/14),78.6％ (11/14), 83.3％ (20/24) and 76.9％ (10/13) in high, moderately, low and undifferentiated gastric carcinomas, respectively. MMP 9 was also expressed in two gastric cancer cell lines and in tumor associated fibroblasts. Conclusions: MMP 9 can be produced by either gastric cancer cells or the fibroblasts cells of tumor stroma. The frequency of MMP 9 expression is closely correlated with tumor development (P更多还原