【摘要】 目的：建立类似临床大肠癌根治术后肝转移动物模型，并建立精确评估肝转移的方法。方法：在裸鼠脾内注入人结肠癌细胞5min后切除脾脏。术后第30天对裸鼠行病理检查并测定肝转移DNA含量。结果：发现肝转移率为100％，肝外其它脏器未见转移结节,组织学证实肝转移结节为低分化腺癌。脾内注入105癌细胞、脾切除组肝转移癌DNA的含量比脾内注入106癌细胞、脾切除组肝转移癌DNA的含量低61.4％。结论：本实验方法建立的模型能很好地模拟临床上大肠癌根治术后肝转移征象，肝转移DNA含量的测定具有准确、可靠的优点。更多还原

【Abstract】 Objective： To establish an animal model imitating clinic sign of liver metastases after curative resection of colorectal cancer and a method of precise evaluation of liver metastases. Methods： The human colon cancer cells (HCCC) were injected into the spleen of BALB/c nude mice, and the spleens were then resected 5 minutes later. On the 30th postoperative day, the nude mice were examined pathologically and the DNA content of liver metastases was determinated. Results： The incidence of liver metastases was found 100％ . No metastatic noudle was observed in extrahepatic organs. Histological examination showed that the metastases were poorly differented colon adenocancer. The DNA content of liver metastases was lower (61.4％ ) in the group given intrasplenic injection of 105 HCCC and subsequent splenectomy than the group of 106 HCCC injection. Conclusion： The experimental model is very good for imitating clinic sign of liver metastases after curative resection of colorectal cancer. The method of deterimenation of DNA content of liver metastases is a precise and reliable means.更多还原