【摘要】 目的 :观察几种在肝癌治疗中常用的药物对肝癌细胞SMMC 772 1端粒酶活性的影响 ,进一步探讨它们的抗癌作用机制。方法 :利用化疗药物顺铂、丝裂霉素C、阿霉素、5 氟尿嘧啶、干扰素、全反式维甲酸、叠氮胸苷处理肝癌细胞 ,然后使用TRAP方法观察处理前后端粒酶活性的变化 ,同时利用流式细胞术观察细胞周期和凋亡。结果 :化疗药物顺铂和丝裂霉素C对肝癌细胞端粒酶活性有抑制作用 ,流式细胞术检测表明当端粒酶活性受到抑制时 ,细胞阻滞在G0 /G1期 ,检测表明端粒酶活性的抑制出现在凋亡之前。干扰素对端粒酶活性亦有抑制作用 ,抑制时细胞阻滞在S期。而阿霉素、5 氟尿嘧啶、全反式维甲酸和叠氮胸苷无抑制作用。结论 :对端粒酶活性的抑制可能是化疗药物顺铂、丝裂霉素C和干扰素发挥抗癌治疗的机制之一。更多还原

【Abstract】 Objective:To investigate the anti cancer mechanism of several drugs that often used in liver cancer treatment by studying its effects on telomerase Methods:The changes of telomerase activity of SMMC 7721 cell following treatment of chemotherapeutic drugs (cisplatin, mitomycin C, doxorubicin, or 5 fluorouracil),interferon, all trans retinoic acid or AZT were observed by using TRAP method We also determined apoptosis and cell cycle by FCM at the same time Results: Chemotherapeutic drugs cisplatin, mitomycin C inhibited telomerase activity FCM results showed that G 0/G 1 were arrested after treatment of cisplatin and mitomycin C when telomerase activity was inhibited, and the inhibition happened before apoptosis Interferon also inhibited the telomerase activity in SMMC 7721 cell, but the cells were arrested in phase S, while doxorubicin, 5 fluorouracil, ATRA and AZT showed no significant inhibition on the telomerase Conclusions: The inhibition of telomerase activity may be one of the mechanisms of anti tumor activity of chemotherapeutic drug cisplatin, mitomycin C and interferon in liver cancer treatment更多还原