【摘要】 提出一种测定牛奶中尿素的新方法 ,以刀豆粉提取液作脲酶催化尿素水解产生氨 ,氨由水扬酸盐光度法测定 .本法中有色化合物的最大吸收波长 70 0nm ,表观摩尔吸光系数ε 70 0 =1.3×10 4 mol-1.L .cm - 1,线性范围 0～ 0 .8μgN/mL ,,检出限 1.1× 10 -6gN/L更多还原

【Abstract】 A new method for determination of urea in milk is presented. Extrat of sword bean powder as urease catalyzes hydrolysis of urea into ammonia, then ammonia is determinated by salicylate spectrophotometry. The maximum absorption wavelength of colored complex lies at 700nm with apparent molar absorption coefficient of 1.3×104mol -1 .L.cm -1 ,the liner range is 0～0.8ugN/mL and sensitivity limit is 1.1×10 -6 gN/L in this method.更多还原