【摘要】 目的 :探讨快速检测C57BL/6Job小鼠基因型方法。C57BL/6Job小鼠是一种ob基因突变种 ,ob基因的点突变导致小鼠遗传性肥胖。该品系广泛用于肥胖及有关疾病研究。方法与结果 :本文用PCR及限制性内切酶法快速 ,准确地检测出C57BL/6J小鼠同窝仔鼠的三种基因型 :野生型 (+/+) ,杂合体型 (ob/+)和突变型 (ob/ob)。结论 :该法只需很少的生物材料 ,即一小段小鼠尾巴 ,适用任何年龄的小鼠 ,对于该品系小鼠的育种、肥胖机理和肥胖预防的研究很有实用意义。更多还原

【Abstract】 Purpose and Methods: The ob gene point mutant in C57BL/6J ob mice cause genetic obesity in all life. To improve the speed and efficiency of ob genotyping, we developed a method with PCR and restriction endonuclease by property primer. Results and Conclusion: Three genotype(ob/ob, ob/+, +/+) of the mouse are rapidly, differetiated with this assay. Since only a small biopsy specimen(tail end) is needed to genotype, the ob mouse at any age is typable and can be used in subsequent breeding or research.更多还原