【摘要】 应用细胞培养和流式细胞仪分析技术分析全蝎水煎液对ＨＬ６０细胞增殖、细胞形态及细胞凋亡的影响。结果全蝎水提物对ＨＬ６０细胞增殖有一定的抑制作用，细胞凋亡比例由对照组的７３６％增加至９４７％（４ｍｇ／ｍｌ）、１２５６％（８ｍｇ／ｍｌ）和２１４４％（１２ｍｇ／ｍｌ）。细胞形态表现为细胞增大，核膜皱缩，核固缩或核碎裂，但细胞膜完整。表明全蝎可诱导ＨＬ６０细胞凋亡，并对细胞增殖有一定的抑制作用更多还原

【Abstract】 In order to study the effect scorpion on the proliferation and induction of programmed cell death(PCD) of HL 60 cells,contral and experimental groups were established.Both of them all pass the examination of FACS after 3 days′ process with 3,6,10mg/ml of scorpion.Results showed that the rate of PCD of contral was 7.36%,and the rate of experimental groups were 9.47%(3mg/ml),12.56%(6mg/ml) and 21.44%(10mg/ml).Some changes in the morphology of experimental groups included:karyopyknosis,shrinkage of the cell membrane and karyorrhexis,and contral groups had no such changes.The inhibition of scorpion on the proliferation of HL 60 cells was also proved and showed definite dose effect relationship.更多还原