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套式PCR法检测输卵管妊娠者的沙眼衣原体感染研究
NestedPCR detection of chlamydia trachomatis infection in patients with tubal pregnancy.
【摘要】 目的:为探讨输卵管妊娠与生殖道沙眼衣原体的关系。方法:采集57例输卵管妊娠者的宫颈分泌物、输卵管组织、胚胎组织,并采集32例卵巢囊肿者的宫颈分泌物、输卵管组织和29例早孕人流者的宫颈分泌物、胚胎组织。应用套式PCR法检测以上标本中的沙眼衣原体DNA。结果:输卵管妊娠者的宫颈分泌物阳性率高达40.4%,明显高于卵巢囊肿者的12.5%和早孕人流者的13.7%(P均<0.05),RR为4.49;输卵管妊娠者的输卵管组织阳性率为24.6%,明显高于卵巢囊肿者的6.3%,RR为4.90;输卵管妊娠者的胚胎组织阳性率为26.3%,明显高于早孕人流胚胎组织的6.9%,RR为4.80。结论:输卵管妊娠与生殖道的沙眼衣原体感染相关。
【Abstract】 Objective:To investigate the vdationship between genital infections with chlamydia trachomatis (ct) and tubal pregnancy.Methods:57 patients with tubal pregnancy (case group),32 patients with ovarian cyst (control group A) and 29 women with early pregnancy(control group B) were investigated as a casecontrol study.NestedPCR technique was used to detect ctDNA in samples from the secretion of cervix,fallopian tube and felal tissue.Results:Case group was more likly to have detectable CtDNA(40.4%)from cervical secretion than control group A and B (pall<0.05),RR was 4.49.The positive rate of case group from fallopian tube was highter than ovarian cyst (p<0.05)and the positive rate from fetal tissue was higher than early pregnancy(p<0.05),RR was 4.90 and 4.80. Conclusion:There is a strong association between tubal pregnancy and genital infection with Ct.
【Key words】 Tubal pregnancy; Chlamydia trachomatis; NestedPCR. (Original article on page 6);
- 【文献出处】 中国优生与遗传杂志 ,CHINESE JOURNAL OF BIRTH HEALTH & HEREDITY , 编辑部邮箱 ,1999年04期
- 【分类号】R714.221
- 【被引频次】1
- 【下载频次】16