【摘要】 为探讨动物红细胞中铅和硒的分布情况以及铅对硒的影响。对摄入铅的小鼠和未摄入铅的对照组小鼠的血液进行了研究。除测定红细胞中铅、硒含量之外，主要还采用分子筛层析的方法以分离红细胞中有关成分，并研究铅、硒在不同成分的洗脱液中的分布情况及其与ＡＬＡＤ的关系。结果在洗脱曲线中，ＡＬＡＤ峰位置上有一个铅峰，硒主峰位于血红蛋白峰之前，摄铅小鼠的样品在血红蛋白位置上的硒含量多于对照组。说明铅与红细胞中ＡＬＡＤ分子之间具有较大的亲和力；在红细胞中多数硒可能主要是与分子量比血红蛋白大的蛋白质结合，但当红细胞中铅增加时，这些硒中的一部分可能会改为与血红蛋白结合更多还原

【Abstract】 To clarify thedistribution of lead and selenium as well as the influence of lead on selenium in animal erythrocytes.The blood of the mice that had assimilated lead and the mice as controls was studied.Some components in the erythrocytes were separated by means of gel filtration;the distribution of lead and selenium in different cfomponents of erythrocytes and its relation to ALAD were studied.The amount of lead and selenium in erythrocytes was measured besides.Result In the chromatographic profile,there was a lead peak corresponding to the ALAD peak,while the main selenium peak was located before the haemoglobin peak,and selenium in position of haemoglobin peak for the mice that had assimilated lead was more than that for the mice as controls.Conclusions:ALAD molecules might have high affinity for lead;most selenium in erythrocytes might mainly be attached to some protein(s) with MW bigger than that of haemoglobin,but some of the selenium might be divorced from the protein(s) and attached to haemoglobin when the lead amount in erythrocytes increased.更多还原