【摘要】 目的观察外源性生长因子和雌激素对离体正常大鼠垂体前叶细胞增殖活性及催乳素（ｐｒｏｌａｃｔｉｎ，ＰＲＬ）基因表达的影响，并探讨生长因子与雌激素作用的关系。方法应用无血清原代培养的大鼠垂体前叶细胞，分别采用荧光染色细胞ＤＮＡ的激光扫描共聚焦显微镜（ｌａｓｅｒｓｃａｎｎｉｎｇｃｏｎｆｏｃａｌｍｉｃｒｏｓｃｏｐｙ，ＬＳＣＭ）扫描、以及原位杂交方法，观察１７－β－雌二醇（ｅｓｔｒａｄｉｏｌ，Ｅ２）、表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈ　ｆａｃ－ｔｏｒ，ＥＧＦ）及转化生长因子β１（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒβ１，ＴＧＦβ１）对大鼠垂体前叶细胞增殖活性和催乳素（ｐｒｏｌａｃｔｉｎ，ＰＲＬ）基因表达的影响。结果生长因子或雌激素作用３６ｈ后，Ｅ２和ＥＧＦ单独作用组细胞ＤＮＡ含量和ＰＲＬｍＲＮＡ含量均较正常对照组明显升高（Ｐ＜０．００１）；两者共同作用上述效应更为显著，高于Ｅ２和ＥＧＦ单独作用组（Ｐ＜０．０１）。ＴＧＦβ１对细胞增殖与ＰＲＬ基因表达均有抑制作用（Ｐ＜０．００１）；而与Ｅ２共同作用时细胞内ＤＮＡ含量和ＰＲＬｍＲＮＡ水平显著高于ＴＧＦβ１单独作用组（Ｐ＜０．００１），但仍低于Ｅ２单独作?更多还原

【Abstract】 Detect the effects of exogenous 17 B-estradiol (E2),epidermal growth factor (EGF),and transforming growth factor B1 (TGFB1 ) on the proliferation and prolactin(PRL) gene expression in primary serum-free cultured anterior pituitary cells in vitro. Methods Laser scanning con focal microscopy (LSCM ) and in situ hybridization in primaryserum-free cultures of rat anterior pituitary cells were employed. Results After 36 hours incubation of the monolayer with E2(10 -8 mol/L) and EGF (10-8 mol/L),DNA and PRL mRNA contents in the cells were increased significantly (P<0. 001 );and when cells were co-incubated with E2 and EGF at the same time,　the levels of DNA and PRL mRNA were higherthan those treated with E, or EGF alone (P<0.01 ), respectively. TGFB1 (2 ng/ml ) treatment decreased the DNA and PRL mRNA contents significantly (P<0. 001 ). Its inhibitoryeffect was reduced at the presence of E2,the DNA and PRL mRNA levels in the cells werehigher than those treated with TGFB1 alone (P<0. 001),but still lower than E2 alone treatment (P<0. 001). Conclusions The results indicate that EGF and TGFB1 exerte stimulatory inhibitory effects,　on cell proliferation and PRL gene expression in anterior pituitary cellsof rats in both hasal and E2-induced conditions. EGF and TGFB1 may be involoved in the regulation of proliferation and PRL gene expression in anterior pituitary cells in vivo; and alsomay be correlated with prolactin-secreting tumors formation induced by E2.更多还原