【摘要】 目的观察１７－β－雌二醇对人脐静脉内皮细胞纤溶因子活性、基因表达以及胞内钙离子渡度（［Ｃａ（２＋）］i）的影响。方法应用发色底物法、细胞原位杂交技术和激光共聚焦扫描显微镜。结果组织型纤溶酶原激活物活性及其基因表达随１７－β－雌二醇浓度的增加而呈剂量依赖性增加；单个内皮细胞胞内钙离子浓度在高浓度雌二醇作用下先上升后下降，生理浓度时降低。结论雌激素可以提高纤溶能力、拮抗胞内Ｃａ(２＋)浓度升高，为雌激素保护心血管作用机制研究提供了部分实验依据。更多还原

【Abstract】 To study the effect of 17 beta-estrodial on fibrinolytic factor in human umbilical vein endothelical cell (HUVEC). Methods When different concentration of 17 betaestrodial including 10-10 mol/L (the group EI ), 10-8 mol/L (the group E2 ), 10-6 mol/L (thegroup E3) were treated in original cultured HUVEC,the changes of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1 ) were measured by spectrophotometric assay,and the level of message RNA were examined by in situ hybridization. Thechanges of intracellular free calcium concentration([Ca2+]) in single cells were observed bykinetic observation with Laster Confocal Microscope. Results The t-PA activity both in E1and E2 were higher than control (P<0. 05 respectively). The t-PA expression in E1,E2,andE3 were increased. The concentration of intracellular free calcium had an elevated and declined curve under the supraphysiological doses of estrodial and declined in incubation with17 beta-eatrodial at 10-6 mol/L. Conclusions These suggeste that the protection of 17 betaestrodial on cardivascular system is associated with high fibrinolytic activity and t-PA expression as well as with the change of [Ca2+].更多还原