【摘要】 目的：黑麦花粉中提取的生物活性肽（ＢＰ）及合成类似活性肽对小鼠、人和ＨＬ６０细胞的作用。方法：用ＭＴＴ法测定ＢＰ与ＨＬ６０细胞共培养７２ｈ时ＨＬ６０细胞的增殖反应，间接免疫荧光测定ＢＰ激活人淋巴细胞产生ＣＤ２５阳性细胞百分率，与ＢＰ共培养的上清液中可溶性白介素２受体（ｓＩＬ２Ｒ）的含量用ＥＬＩＳＡ测定。结果：ＢＰ与ＨＬ６０细胞共培养７２ｈ时ＨＬ６０细胞的增殖反应，ＭＴＴ测定ＯＤ值为０．３３９±０．０１５，未加ＢＰ的对照ＯＤ值０．２７９±０．０３７（Ｐ＜０．０５）。间接免疫荧光测定ＢＰ激活人淋巴细胞产生ＣＤ２５阳性细胞百分率为（５３．９７±７．１２）％（浓度１μｇ／ｍｌ）和（４２．５３±４．４）％（浓度１０μｇ／ｍｌ），对照（１１．７７±１．８８）％（Ｐ＜０．００１）。与ＢＰ共培养的上清液中ｓＩＬ２Ｒ的含量用ＥＬＩＳＡ测定为（１７８２．３±７８．１８）ｕ／ｍｌ，低于对照（２３８１．２±９９．９）ｕ／ｍｌ。结论：ＢＰ有刺激ＨＬ６０细胞增殖的作用，ＢＰ对淋巴细胞有激活作用。我们讨论了ＢＰ的作用机制及其发展成新的免疫调节剂的可能性。更多还原

【Abstract】 OBJECTIVE :The effect of bioactive peptide (BP) buckwheat and its synthetic analogue on murine and human lymphocytes and HL60 cells. METHODS :The proliferative response of HL60 obtained from 72 h coculture of BP and HL60 cells was determined by MTT method;the CD25 positive cells rate produced by BPactivated human lymphocyte was measured by indirect immunofluorescence;and the sIL2R content in the supernatant of cocultured medium was determined by ELISA. RESULTS :In determination of the proliferative response,an optical density (OD)of 0.3990.015 for sample was obtained versus an OD of 0.2790.037 for blank;the CD25 positive cells rate obtained were (53.977.21)%(1 g/ml)and(42.534.4)%(10 g/ml);and the content of sIL2R in supernatant of cocultured medium was (1782.378.18)u/ml lower than control of (2381.299.9)u/ml. CONCLUSION :From the results obtained,it can be concluded that BP has an effect to stimulate the proliferative response of HL60 cells and that lymphocytes can be activated by BP.The mechanism o更多还原