【摘要】 目的：研究同源盒ＨＯＸ基因的表达在ＡＴＲＡ诱导白血病细胞分化中的作用。方法：利用我们建立的一种高效、简单的ｃＤＮＡ克隆方法－ＨＯＸ家庭基因指纹图技术，克隆ＨＬ－６０细胞内的ＨＯＸｃＤＮＡ基因。采用半定量ＲＴ－ＰＣＲ技术初步探讨ＡＴＲＡ对所克隆的ＨＯＸ基因表达的影响。结果：筛选出一个在ＨＬ－６０细胞内有表达的同源盒基因一ＨＯＸＡＩ基因ｃＤＮＡ片段，初步研究证实，此基因的ｍＲＮＡ水平在ＡＴＲＡ的作用下被上调。结论：ＡＴＲＡ可诱导ＨＬ－６０细胞内ＨＯＸＡ１基因表达增强。提示在ＡＴＲＡ诱导细胞分化而治疗恶性肿瘤的分子机制中，包括上调ＨＯＸＡ１基因表达而致白血病细胞分化成熟。更多还原

【Abstract】 Objective: To investigate the expression and the role of HOX genes in leukemic cells differentiation induced by all-trans retinoicacid (ATRA). Methods: mRNA fingerprinting which was established by us before for HOX familiar genes, was a rapid and efficientcDNA cloning method. Using mis technique, we tried to clone some HOX cDNA genes. Using semi-quantitative reverse transcriptionpolymerase chain reaction technique, we tried to detect the expression levels of these genes in HL-60 cells induced by ATRA.Results: A cDNA fragment from Homeobox HOXA1 gene that was one of homeobox familiar genes was cloned. The expressionlevel of HOXA1 gene in HL-60 cells induced by ATRA increased compared with that of the control. Conclusions: ATRA canupregulate the expression of HOXA1 gene in HL-60 cells. It suggests that the upregulation of HOXA1 gene expression beinvolved in the differentiation of leukemic cells.更多还原