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2,4-D对甘薯体细胞胚胎发生的调控
2,4 D Regulated Somatic Embryogenesis of Sweet Potato
【摘要】 将来源于‘徐薯18’叶片的胚性愈伤组织,接种在含有不同2,4D浓度的液体MS培养基中进行悬浮培养,悬浮细胞表现出不同的形态结构、分裂方式和发育途径:2,4D浓度为1mg/L时,细胞均等分裂,增殖迅速;不含2,4D时,细胞多进行不均等分裂,并发育成体细胞胚。不同2,4D浓度中培养的悬浮细胞,其胞外过氧化物同工酶谱及其随时间变化的方式有很大差异,并与细胞的生长、发育过程密切相关
【Abstract】 Divergences were discovered in cell morphology, mode of cell division and passage of cell differentiation, when embryogenic calli induced from leaves of `Xushu 18’ sweet potato were suspension_cultured in modified MS medium with different concentrations of 2,4 D. Cells grew quickly and divided equally in medium containing 1 mg/L 2,4 D. When 2,4 D was deleted, the cells divided unequally and developed into embryos. Extracellular peroxidase isozyme patterns changed greatly during suspension culture with different concentrations of 2,4 D and were closely related to cell development process.
【Key words】 Sweet potato; Somatic embryogenesis; Suspension culture; Peroxidase isozyme;
- 【文献出处】 植物学通报 ,CHINESE BULLETIN OF BOTANY , 编辑部邮箱 ,1999年04期
- 【分类号】S531
- 【被引频次】16
- 【下载频次】175