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2,4-D对甘薯体细胞胚胎发生的调控

2,4 D Regulated Somatic Embryogenesis of Sweet Potato

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【作者】 崔红丁群星桂耀林郭仲琛

【Author】 CUI Hong 2DING Qun Xing 2GUI Yao Lin 2GUO Zhong Chen 1(Biology Department, Xiamen University, Xiamen 361005) 2(Institute of Botany, The Chinese Academy of Sciences, Beijing 100093)

【机构】 厦门大学生物系!厦门361005中国科学院植物研究所!北京100093

【摘要】 将来源于‘徐薯18’叶片的胚性愈伤组织,接种在含有不同2,4D浓度的液体MS培养基中进行悬浮培养,悬浮细胞表现出不同的形态结构、分裂方式和发育途径:2,4D浓度为1mg/L时,细胞均等分裂,增殖迅速;不含2,4D时,细胞多进行不均等分裂,并发育成体细胞胚。不同2,4D浓度中培养的悬浮细胞,其胞外过氧化物同工酶谱及其随时间变化的方式有很大差异,并与细胞的生长、发育过程密切相关

【Abstract】 Divergences were discovered in cell morphology, mode of cell division and passage of cell differentiation, when embryogenic calli induced from leaves of `Xushu 18’ sweet potato were suspension_cultured in modified MS medium with different concentrations of 2,4 D. Cells grew quickly and divided equally in medium containing 1 mg/L 2,4 D. When 2,4 D was deleted, the cells divided unequally and developed into embryos. Extracellular peroxidase isozyme patterns changed greatly during suspension culture with different concentrations of 2,4 D and were closely related to cell development process.

  • 【文献出处】 植物学通报 ,CHINESE BULLETIN OF BOTANY , 编辑部邮箱 ,1999年04期
  • 【分类号】S531
  • 【被引频次】16
  • 【下载频次】175
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