【摘要】 用水稻脂质转移蛋白（ｌｉｐｉｄｔｒａｎｓｆｅｒｐｒｏｔｅｉｎ，ＬＴＰ）的ｃＤＮＡ（ｐＦＤＲＳＣ１１０）为探针，从水稻（ＯｒｙｚａｓａｔｉｖａＬ．ｓｐ．ｉｎｄｉｃａ）“广陆矮４号”基因文库中筛选出ＬＴＰ基因，完成了１．８ｋｂ长片段的序列测定。该基因编码一个１２１个氨基酸组成的肽，编码区中间有一个９０ｂｐ的内含子，基因的调控区除有２个ＴＡＴＡｂｏｘ和ｐｏｌｙＡ加工信号外，还存在多个回文序列和逆向重复序列。该基因编码的蛋白质Ｎ端是由２８个氨基酸组成的信号肽，同源性比较表明它具有典型的植物ＬＴＰ特征。更多还原

【Abstract】 A lipid transfer protein gene, LTP 110, was isolated from rice ( Oryza sativa L. ssp. indica ) genomic DNA library using a LTP cDNA(pFDRSC110) as probe. Sequence analysis indicated that it was 1.8 kb in length and encoded a peptide of 121 amino acids. The coding region was interrupted by an intron of 90 bp. There were two canonical TATA boxes, several inverted and repeated elements and palindromic sequences at the 5′ upstream and a polyadenylation signal at the 3′ downstream. A signal peptide of 28 amino acids was found at its N_terminus. Homology comparison revealed that it possessed the typical features of plant LTPs.更多还原