【摘要】 玫瑰果（Ｒｏｓａｒｕｇｏｓａｖａｒ．ｃｈａｍｉｓｏｎｉａｎａ）果实中有一种具有高ＳＯＤ活性的成分，系统研究表明，这是一个小肽，定名为类ＳＯＤ物质。用有机溶剂分级、聚几内酰胺、ＤＥＡＥ１１、Ｚｅｒｏｌｉｔ２２５柱分离纯化，提纯物经紫外光谱、圆二色谱分析、氨基酸分析、邻菲咯啉定铁、ＤＮＳＣｌ法检测末端氨基酸等实验，证实类ＳＯＤ物质的等电点为５．２，每分子肽含一个Ｆｅ原子，有７０个氨基酸左右，分子量约为７０００，可能是一种结合多肽，末端氨基酸被封闭，富含ｃｙｓ，呈无规卷曲状态，这与其很高的温度稳定性及ｐＨ稳定性是一致的。更多还原

【Abstract】 It is known that SOD activity in rose (Rosa rugosa var. chamissoniana) fruit is very high. By organic solvent fractionation and dialysis, the authors found that the substance of scavenging O -· 2 is mainly a material with low molecular weight (termed as SOD like material). Ultraviolet spectrophotometry, CD spectra, amino acids analysis and iron determination of the purified peptide indicated that it has approximately 70 amino acids and one atom of iron per molecule. The cys content of SOD like material is higher than that of SOD. In the ultraviolet region, a peak at 280 nm was observed. CD spectra showed a characteristic peak of random coil. The N terminal group of this molecule, as determined by Pansyl Chloride method, was not enzymatically cleaveable. This SOD like material also has very high temperature and acidic stability.更多还原