节点文献
玉米中哺乳动物细胞凋亡相关基因rb同源序列的检出及其荧光原位杂交
Detection and Fluorescence in Situ Hybridization of Homologous Sequences for Mammalian Gene rb Related to Apoptosis in Maize
【Abstract】 HumangenerbrelatedtoapoptosiswasusedastheprobefortheSouthernblothybridizationofthe genomicDNAinbothmaize (ZeamaysL .)andrice (OryzasativaL .) .Theresultsindicatedthattheho mologoussequencesofrbwerepresentedinthetwospecies .Thephysicallocationoftherbhomologousse quenceswasalsocarriedoutinmaizechromosomesbyfluorescenceinsituhybridization (FISH) .Thegenerb washybridizedontothelongarmsofthechromosomes 5and 6 ,andtheshortarmofthechromosome 8.The detectionratesofFISHwere 7.58% ,16 .16 %and 10 .10 % ,andpercentdistancesfromcentromeretothe detectionsiteswere 86 .17± 3.2 2 ,94 .10± 2 .59and 92 .4 7± 2 .33respectively .Theseresultsprovidedim portantcluestofurtherresearchofplantapoptosisgenes .
【关键词】 玉米;
细胞凋亡;
rb;
同源序列;
荧光原位杂交;
【Key words】 Maize; Apoptosis; rb; Homologoussequences; Fluorescenceinsituhybridization;
【Key words】 Maize; Apoptosis; rb; Homologoussequences; Fluorescenceinsituhybridization;
- 【文献出处】 植物学报 ,ACTA BOTANICA SINICA , 编辑部邮箱 ,1999年12期
- 【分类号】S188
- 【被引频次】4
- 【下载频次】51