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小麦-簇毛麦易位系 6VS/6AL中6VS的遗传传递及其所携Pm21基因的遗传稳定性分析

Transmission of 6VS Chromosome in Wheat-Haynaldia villosa Translocation Lines and Genetic Stability of Pm21 Carried by 6VS 

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【作者】 刘金元; 陶文静; 刘大钧; 陈佩度; 李万隆; 向齐军; 段霞瑜;

【Author】 LIU Jin_Yuan  TAO Wen_Jing  LIU Da_Jun  CHEN Pei_Du LI Wan_Long (Key Open Laboratory of Agricultural Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing 210095) XIANG Qi_Jun DUAN Xia_Yu (Insti

【机构】 南京农业大学农业部作物细胞遗传重点开放实验室!南京210095; 南京农业大学农业部作物细胞遗传?;

【摘要】 以簇毛麦(Haynaldiavillosa(L.)Schur)物种专化重复序列探针pHv62及小麦(TriticumaestivumL.)第六部分同源群短臂专化RFLP探针Psr113对扬94138(“扬麦158”的改良品系)与易位系92R149配制的F2群体进行分析,观察到易位系中的6V短臂在杂交后代中的传递率为69.5%,接近75%的理论值。对来自同一个F1的另外147株F2群体以6个白粉菌菌株进行苗期抗病性测定,检测结果表明6VS上所携Pm21基因在向“扬麦158”小麦基因型转移时,按显性单基因遗传,并能很好地表达。

【Abstract】 F 2 plants from Yang94138×92R149 were analyzed using speciesspecific probe pHv62 for Haynaldia villosa (L.) Schur and RFLP probe Psr113 located on the short arm of homoeologous group six for wheat ( Triticum aestivum L.). The results showed that the transmission rate of 6VS in the F 2 was 69.5%, which was close to the expected value of 75%. 147 F 2 plants from the above F 1 plant were tested for their resistance in seedling stage to 6 pathogenic strains of powdery mildew. It was observed that Pm 21 genes on 6VS inherited dominantly and expressed well when transferred to wheat—“Yangmai 158” genotype.

【关键词】 簇毛麦; Pm21基因; 小麦; 传递率; RFLP探针;
【Key words】 Haynaldia villosa; Pm 21 gene; Wheat; Transmission rate; RFLP probe;
【基金】 国家自然科学基金!(No .3 9770 413 );国家“86 3”计划资助项目!(No .Z_17_0 4_0 1)
  • 【文献出处】 植物学报 ,ACTA BOTANICA SINICA , 编辑部邮箱 ,1999年10期
  • 【分类号】Q943
  • 【被引频次】42
  • 【下载频次】197
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