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小麦-簇毛麦易位系 6VS/6AL中6VS的遗传传递及其所携Pm21基因的遗传稳定性分析

Transmission of 6VS Chromosome in Wheat-Haynaldia villosa Translocation Lines and Genetic Stability of Pm21 Carried by 6VS 

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【作者】 刘金元陶文静刘大钧陈佩度李万隆向齐军段霞瑜

【Author】 LIU Jin_Yuan  TAO Wen_Jing  LIU Da_Jun  CHEN Pei_Du LI Wan_Long (Key Open Laboratory of Agricultural Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing 210095) XIANG Qi_Jun DUAN Xia_Yu (Insti

【机构】 南京农业大学农业部作物细胞遗传重点开放实验室!南京210095南京农业大学农业部作物细胞遗传?

【摘要】 以簇毛麦(Haynaldiavillosa(L.)Schur)物种专化重复序列探针pHv62及小麦(TriticumaestivumL.)第六部分同源群短臂专化RFLP探针Psr113对扬94138(“扬麦158”的改良品系)与易位系92R149配制的F2群体进行分析,观察到易位系中的6V短臂在杂交后代中的传递率为69.5%,接近75%的理论值。对来自同一个F1的另外147株F2群体以6个白粉菌菌株进行苗期抗病性测定,检测结果表明6VS上所携Pm21基因在向“扬麦158”小麦基因型转移时,按显性单基因遗传,并能很好地表达。

【Abstract】 F 2 plants from Yang94138×92R149 were analyzed using speciesspecific probe pHv62 for Haynaldia villosa (L.) Schur and RFLP probe Psr113 located on the short arm of homoeologous group six for wheat ( Triticum aestivum L.). The results showed that the transmission rate of 6VS in the F 2 was 69.5%, which was close to the expected value of 75%. 147 F 2 plants from the above F 1 plant were tested for their resistance in seedling stage to 6 pathogenic strains of powdery mildew. It was observed that Pm 21 genes on 6VS inherited dominantly and expressed well when transferred to wheat—“Yangmai 158” genotype.

【关键词】 簇毛麦Pm21基因小麦传递率RFLP探针
【Key words】 Haynaldia villosaPm 21 geneWheatTransmission rateRFLP probe
【基金】 国家自然科学基金!(No .3 9770 413 );国家“86 3”计划资助项目!(No .Z_17_0 4_0 1)
  • 【文献出处】 植物学报 ,ACTA BOTANICA SINICA , 编辑部邮箱 ,1999年10期
  • 【分类号】Q943
  • 【被引频次】42
  • 【下载频次】197
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