【摘要】 以安替比林为参照物，建立体外人胎盘灌流技术，采用局部胎盘小叶的开放及封闭灌流方法。开放灌充安替比林消除率与时间、母体循环中的浓度无显著相关性，只随流速比的增大而增大，二者之间有显著相关性（ｒ＝０９０６）。封闭灌流两个方向的灌流，供体面安替比林浓度逐渐降低，且都符合指数函数Ｃ（ｔ）＝Ｃｏ×ｅ－ｋｔ，受体面浓度逐渐升高。两个方面的清除率分别为０４１ｍｌ／ｍｉｎ，０５２ｍｌ／ｍｉｎ，两者间无显著差异，提取效率母体面到胎儿面方向略低（Ｐ＜００５），分别是６８３％，２８９％。以上结果与相关资料一致，说明本室体外人胎盘灌流设备、技术均已完备，结果可靠。更多还原

【Abstract】 The technique of transfer across the perfused human placenta in vitro was set up and assessed by studying of Antipyrine.We used open perfusion and closed perfusion in a cotyledon in our experiment.In the open perfusion there was no significant relation between the clearance rate of Antipyrne and perfusion time as well as the concentration in matemal perfusate.The clearance rate increased when the flow rate ratio increased and there was significant reation between them (r=0 906).In closed perfusion we performed two direction perfusion.It was showed that the concentration of Antipyrine decreased gradually in supplying side and the data of each experiment was fitted to the exponential function C (t) =C o×e -kt ,however th concentration of Antipyrine in collecting side increased gradually.The clearance rate was 0 41,0 52 ml/min respectively and there was no significant difference.We also concluded that the efficiency of extractions from matemal side to fetal side was 2 98%.It was lower than that from fetal to matemal 6 83%(P<0 05).All our conclusions were consistent with that of other researchers,so the instruments and technique of perfused human placenta in vitro proved valid and the results obtained were credible.更多还原