【摘要】 大麦叶片液泡膜微囊经Ｈ２Ｏ２ 和·ＯＨ处理后，Ｈ＋ＡＴＰａｓｅ 和Ｈ＋ＰＰａｓｅ 水解活性和泵运质子活性下降， Ｈ＋ＰＰａｓｅ 对Ｈ２Ｏ２ 更敏感一些。同时加入与Ｈ２Ｏ２ 和·ＯＨ 相同浓度的抗坏血酸（ＡｓＡ） 或甘露醇可显著恢复两种酶的活性和质子转运活性。Ｈ２Ｏ２ 和·ＯＨ 处理后，Ｈ＋ＡＴＰａｓｅ 米氏常数（ Ｋｍ） 变大，Ｈ＋ＰＰａｓｅ 的最大反应速度（ Ｖｍａｘ） 减小更多还原

【Abstract】 Both hydrolytic and H + transport activities of H + ATPase and H + PPase in the tonoplast vesicles isolated from barley leaves were lowered by H 2O 2 and ·OH treatments. The hydrolytic activity of H + PPase was more sensitive to H 2O 2 than that of H + ATPase (Figs.1,2,4,5). The depressed hydrolytic and H + transport activities of these two enzymes were recovered significantly by the addition of AsA, mannitol at the same concentration as H 2O 2 and ·OH . K m value for H + ATPase was increased and V max of H + PPase decreased by treatments with H 2O 2 and·OH (Fig.3).更多还原