【摘要】 目的了解ＴＴＶ感染状况，探讨ＴＴＶ致病性及对干扰素治疗的应答。方法采用ＰＣＲ和原位杂交方法检测血清和肝组织中ＴＴＶ ＤＮＡ，回顾分析８例ＴＴＶ／ＨＣＶ感染者干扰素治疗后病毒及ＡＬＴ应答状况。结果各型肝病患者（３６．７％～４２．８％）和职业供血员（４３．２％）ＴＴＶ感染率均显著高于义务供血员（健康对照人群，５．６％）。部分感染者采用斑点杂交方法可自血清中检出ＴＴＶ ＤＮＡ（３／３０，１０．０％），提示存在较高的血清病毒负荷， １６例慢性非甲～戊型肝炎患者及１６例肝炎后肝硬化患者肝组织以原位杂交检测，各６例ＴＴＶ ＤＮＡ阳性（３７．５％）。 ８例ＴＴＶ／ＨＣＶ混合感染者经２４周疗程干扰素治疗后，６例维持持久ＡＬＴ应答（停药后２４周ＡＬＴ正常），治疗１２周时， ８例血清ＴＴＶ ＤＮＡ均阴转，但 ２例停药后复阳， ＡＬＴ无应答的２例患者， ＨＣＶ ＲＮＡ呈阳性但ＴＴＶ ＤＮＡ阴怀；而ＴＴＶ ＤＮＡ阳转的２例患者ＡＬＴ均维持应答。结论 ＴＴＶ感染在肝病患者和职业供血员中较普遍， ＴＴＶ可在肝组织中活跃复制，其对干扰素治疗反应较敏感，ＴＴＶ／ＨＣＶ重叠感染时ＴＴＶ无明显致肝损伤作用。更多还原

【Abstract】 Objective Investigate the prevalence of TTV infection and also evaluate its pathogenicity and response to interferon therapy. Methods TTV DNA in sera and liver tissues were detectedduring IFN-α therapy and were retrospectively in patients with chronic hepatitis C superinfected with TTV who received a regime of 24-week IFN-α treatment. Results The prevalence of TTV infection in patients with liver diseases (36.7%-42.8%) and occupational blood donors (health control, 5.6%). TTV DNA in sera of some subjects could be detected with dot blot method (3/30, 10.0%), indicating a relatively higher serum viral load in these subjects. With in situ hybridezation detection, TTV DNA was found in liver in 37.5% patients with non-A to E chronic hepatitis and in patients with posthepatic cirrhosis, respectively. Among 8 patients with hepatitis C virus (HCV) and TTV superinfection, 6 had sustained ALT response after 24 weeks interferon therapy (ALT remained normal within 24 weeks after of IFN withdrawal. TTV DNA was seronegative in all 8 cases at week 12th of IFN treatment. 2 cases, however,were TTV DNA seropositive again after the therapy ceased. Both of the 2 patients without sustained ALT response were TTV DNA seronegative but HCV RNA seropositive. On the other hand, the 2 patients with TTV DNA seropositive again after withdrawal of IFN had ALT responses. Condusion TTV infechon is common in patients with liver diseases and occupational blood donors. TTV may replicate actively in liver. It seems that TTV has no pathogenetic effect on the liver in patients with super infected HCV/TTV TTV seems to be sensihve to IFN therapy.更多还原