【摘要】 用纯化的猪囊尾蚴囊液蛋白作诊断抗原，吸附于硝酸纤维素膜片上，经４０％ 乙醇固定，以斑点酶联免疫吸附法检测猪囊虫病抗体。结果发现：在一抗、二抗和底液中加入１０ｍ Ｍ ＥＤＴＡ，四甲基联苯胺作显色剂，１ｈ 内即可完成诊断，并显著提高免疫诊断的灵敏性和特异性（９９％ ）。据此研制的免疫诊断试剂盒，具有快速、灵敏、特异和简便的特点，４℃存放１ 年、室温８ 个月内均有效。应用该技术检测凉山州昭觉县囊虫病疫区７００头份猪血清，发现囊虫病阳性率达３０％ 。更多还原

【Abstract】 The purified proteins from cysticercus fluid were absorbed on nitrocellulose membrane,fixed by 40% alcohol and then used as immunodiagnostic antigen to detect the antibody against pig cysticercosis by Dot ELISA.The result shows that when 10mM EDTA was added into the dilution buffer of the primary and secondary antibody and substrate liquid,TMB as colouring reagent,the diagnostic process of Dot ELISA could be finished in one hour,and the immunodiagnosis specificity and sensitivity were obviously raised.The immunodiagnostic kit prepared as the method mentioned above was specific,sensitive and easy to use,it was still effective after stored for one year at 4℃ or eight months at room temperature.700 sera of pigs from Zhaojiao county of Liangshan were diagnosed with the immunodiagnostic kit,and 30% of them were positive for cysticercosis antibody.更多还原