【摘要】 用表达马立克氏病病毒(MDV)Ⅰ型疫苗株CVI988/Rispens 糖蛋白B(gB)基因的重组鸡痘病毒(rF-PV-gB/R)和鸡痘病毒(FPV)疫苗株282E4 免疫1 日龄不同品种的鸡,来评价2 种病毒株的安全性及免疫性。试验结果表明,rFPV gB/R和FPV 282E4 免疫鸡后对FPV 强毒攻击均可提供100% 的保护,对有FPV母源抗体的商品蛋鸡安全无毒性,而对无母源抗体的SPF鸡的毒性则不同。斑点杂交和Southern 杂交证实,rFPV-gB/R 中无氨苄青霉素的抗性基因。更多还原

【Abstract】 Recombinant fowlpox virus (rFPV) was constructed to express glycoprotein B (gB) gene from CVI988/Rispens strain of Marke′s disease virus(MDV). The immunity and safety in different chickens were evaluated after inoculation with rFPV gB/R or wild FPV on 1 day old. The results showed that the complete protection against challenge with virulent FPV was observed. Both rFPV gB/R and FPV were safe to commerical production chickens with maternal Ab + to FPV, as compared to SPF chickens of different genetic background. It has been demonstrated that there was no ampicillin resistant gene in rFPV gB/R.更多还原