【摘要】 目的:探讨急性心肌缺血再灌注早期不同时间心肌细胞内Fos蛋白表达的变化,为心肌早期缺血再灌注损伤致死死后诊断提供新方法。方法:在32只SD大鼠建立心肌早期缺血再灌注损伤模型,另外40只大鼠分为正常与缺血对照组。用免疫组化SABC法结合图象分析研究心肌细胞核Fos蛋白的累积情况。结果:缺血30min再灌注30min后,再灌注区有部分心肌细胞核呈弱阳性着色,以后随缺血时间延长核阳性增强。缺血90min再灌注30min后核棕褐色染色最强,180min后又开始减弱。正常和单纯缺血组心肌细胞核未见有阳性反应。HE染色无明显病理改变。结论:Fos蛋白表达高峰在缺血90～180min之间,FosSABC染色法可诊断缺血30min再灌注30min或更长时间的损伤。免疫组化染色法检测心肌细胞核Fos蛋白的表达有望成为急性心肌缺血再灌注死后诊断的一种有意义的手段。更多还原

【Abstract】 Objective: To investigate the changes of Fos protein induced by myocardial ischemia/reperfusion (MI/R) during acute period, and provide a new method for postmortem diagnosis of acute ischemia/rperfusion. Methods: It was established that the model of MI/R successfully in 32 anaethetized SD rats. Parallelly, the other 40 rats were used as normal and ischemic control. Immunohistochemical SABC method and computerized image analysis were used to observe alterations of Fos protein in nuclei of cardiac myocytes. Results: After 30 min ischemia followed by 30 min reperfusion, the area of MI/R showed a part of nuclei of cardiac myocytes weak staining, and became stronger with the elongation of ischemia. But after 90 min ischemia followed by 30 min reperfusion, staining of nuclei of cardiac myocytes was strongest, it began to attenuate after 180 min. The myocardium in normal and ischemic control animals showed negative staining. No changes were seen with HE staining in all groups. Conclusion: There is a high point of Fos protein expression during the period of 90 to 180 min. SABC immunohistochemical method may reveal injury of myocardial 30 min ischemia/30 min reperfusion or longer time. Immunohistochemical detection of Fos protein in nuclei of cardiomyocytes would be a meaningful tool for the postmortem diagnosis of myocardial acute ischemia/reperfusion.更多还原