【摘要】 目的:观察Leptin对大鼠肝细胞葡萄糖氧化的影响,及其剂量与时间效应。方法:培养肝细胞以不同剂量Leptin处理,以及以同一剂量Leptin处理不同时间,以液体闪烁计数法检测肝细胞氧化D[U14C]葡萄糖的量。结果:细胞培养1h,当Leptin为10μg·L-1时,肝细胞葡萄糖氧化与未予Leptin处理的对照组之间无明显差异,当Leptin为50μg·L-1,葡萄糖氧化较对照组下降(P<005),在50～200μg·L-1范围随剂量的上升而逐渐下降。以Leptin100μg·L-1延长作用时间至3h、12h、24h、48h,葡萄糖氧化反而较1h时显著上升(P<001),恢复至对照组水平。结论:低剂量Leptin对肝细胞葡萄糖氧化无显著影响,高剂量Leptin对肝细胞葡萄糖氧化具剂量依赖性急性抑制作用,慢性作用时,其抑制作用消失。更多还原

【Abstract】 Objective: To observe the dose and time effects of leptin on glucose oxidation in cultured rat hepatic cells. Methods: Hepatic cells were incubated with leptin of different doses and different time courses with a same dose. D [U 14 C]glucose oxidation were determined by liquid scintillation counting. Results: Leptin treatment of 10 μg·L -1 for 1h had no significant effects on glucose oxidation in hepatic cells. However, at the dose of 50 μg·L -1 , leptin significantly inhibited glucose oxidation vs control oxidation (P<0 05). At the doses range from 50 μg·L -1 to 200 μg·L -1 , the inhibitory effect of leptin was dose dependent. This effects were reversed or glucose oxidation was slightly higher than the control group when hepatic cells were exposed to leptin(100 μg·L -1 ) for 3 h、12 h、24 h and 48 h. And comparing to the 1 h group the glucose oxidation were significantly elevated (P<0 01). Conclusion: Low dose of leptin has no significant effect on glucose oxidation in hepatic cells. A relatively high dose of leptin has an acute, dose dependent inhibitory effect on the glucose oxidation in hepatic cells. This effect is reversed by prolonging the exposure time.更多还原