【摘要】 本文报道１ 例临床狂犬病病例， 男性２２ 岁， 发病３ 天出现三恐症状， 继而四肢撑于床上， 口中流涎， 呼吸循环衰竭死亡。追述病史， ８ 岁时曾被犬咬伤左臂， 留有疤痕， 当时未作处理， 以后未密切接触过犬或与犬类有关的食品和物品。为确诊长潜伏期的狂犬病， 采取一系列实验研究， 采集死者脑海马回， 应用单克隆抗体 （ Ｍｃ Ａｂ） 作酶联免疫反应和免疫荧光检测病毒抗原， 仅几小时即检测到病毒抗原， 达到快速诊断。为确证检测结果， 用细胞培养和动物接种分离病毒，获得病毒株， 并进一步鉴定。从而为长达１４ 年潜伏期的狂犬病病例找到了病原， 对防病措施的落实提供了依据。更多还原

【Abstract】 A 22 years old rabies patient who was afraid of wind,water,light,and was died of circulatory failure.14 years ago he was bitten by a dog on left arm remaining a scar and did not inject rabies vaccine. \ \ In order to determine the rabies patient,a series of experiments were carried out.The brain was collected and the brain smear was made.ELISA and IFA were detected by McAb.In a couple of hours the rabies virus antigen were found.Meanwhile the virus isolate were tested by inoculation of brain suspension into N 2A cell culture and mice brain. After 48 hours of inoculation,the evidence of positive result was shown in the cell plasm by IFA and the titer was 10 5 .The rabies virus strain was obtained by mice isolation in 2 passages.These experiment results confirmed a rabies patient with long incubation period.更多还原