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应用ELISA检测阿米巴脓抗原和循环抗原诊断阿米巴肝脓肿的价值

DETECTION OF ENTAMOEBA HISTOLYTICA ANTIGEN IN LIVER PUS AND SERUM WITH ELISA FOR THE AETIOLOGICAL DIAGNOSIS OF AMEBIC LIVER ABSCESS

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【作者】 安亦军; 郭增柱; 谢云秋;

【Author】 An Yijun; Guo Zengzhu ;Xie Yunqiu(Beijing TrOPical Medicine Research Institute,Beijing, 100050)

【机构】 北京热带医学研究所!北京; 100050; 湖南省耒阳市人民医院;

【摘要】 应用ELISA法检测阿米巴肝脏肿患者脓液和血清标本中的阿米巴抗原。结果42例患者中有41例植出脓抗原,检出率为97.6%,其中包括8例镜检阿米巴滋养体阳性和33例镜检阴性者;有39例检出循环抗原,检出率为92.9%。脓抗原阳性反应强度高于循环抗原者(P<0.05)。各种对照标本的阿米巴抗原植出率为0。提示可用ELISA抗原检测法替代镜检和培养法进行阿米巴肝脓肿的病原学诊断。

【Abstract】 ELlSA test was used to diagnose amebic liver abscess by detecting Entamoeba histolytica antigen in liver as-pirates and serum. The antigens were demonstrated in 4l (97’ 6 % ) of 42 liver aspirates from the patients with amebic liver ab-scess. It was positive that the 8 cases showed Entamoeba histolytica trophozoites in the"pus" and 33 samples negative for theparasite by smear. None of the controls was a false positive reaction obtained. Furthermore,it had been shown that amebic in-fection was followed by the appearance of specific antigen or antigenic substances in the serum which were demonstrated in 39(92. 7 % ) of 42 proved cases of amebic liver abscess. The results suggest that the detection of amebic antigen in pus and serumwith ELISA is a sensitive and useful method for the aetiologica1 diagnosis of amebic liver abscess.

  • 【文献出处】 中国人兽共患病杂志 ,CHINESE JOURNAL OF ZOONOSES , 编辑部邮箱 ,1999年03期
  • 【分类号】R531.1
  • 【被引频次】2
  • 【下载频次】47
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