【摘要】 用经过筛选的１１ 个籼粳分化特异探针对培矮６４Ｓ、Ｎ４２２Ｓ、１０８Ｓ、ＬＳ２Ｓ等４ 个不育系和５７ 份恢复系的ＤＮＡ 籼粳分化程度进行了初步研究。结果表明，供试材料ＤＮＡ 在籼粳分化程度上存在明显差异，表现在其ＴＤｊ值的分布范围为０．０６７４～０．８８６４。大多数品种籼粳分化比较彻底，ＤＮＡ 上处于典型籼粳之间的中间型的材料很少。培矮６４Ｓ、中４１３、特青２ 号、桂朝２号、晚三、密阳２３ 等品种的ＴＤｊ值小于０．１，属于典型籼稻；优良三系杂交籼稻恢复系明恢６３ 和密阳４６ 虽然在分类上属于籼稻，但都含有一定粳稻成分。ＬＳ２Ｓ、零轮、Ｃ４１８２、Ｃ４１８、Ｃ４１１、中作９２９９、中作９４６、中花３ 号、Ｍａｒｓ等都为偏粳，它们的ＴＤｊ值均小于秋光、中作９３ 等典型粳稻。通过部分品种的ＤＮＡ 的ＴＤｊ值与同工酶ＴＤｊ值比较研究发现，二者结果基本一致，但ＤＮＡ ＴＤｊ值提高了检测不同品种遗传分化程度的灵敏度。更多还原

【Abstract】 The degrees of indica japonica differentiation of four photosensitive and thermosensitive genetic male sterile lines, such as Peiai64S, N422S, 108S and LS2S, and 57 restorer lines were investigated by RFLP analysis of 11 specific probes of indica japonica differentiation. It indicated that there was obvious difference in the degree of indica japonica differentiation among the varieties, and TDj values (A parameter stands for the probability of a japonica variety) of the used materials ranged from 0.0674 to 0.8864 . Most of the used varieties showed complete indica japonica differentiation, while a few of the varieties were the middle types between typical indica and japonica. The TDj values of Peiai64S, J413, Teqing 2, Guichao 2, Wan 3, Milyang 23 were less than 0.1, belonging to typical indica type. Minghui 63 and Milyang 46,the excellent restorer lines of three line hybrid rice, belonged to indica type, but they had some japonica genes in their genomes. LS2S, Linglun, C418, C411, Zhongzuo 9299, Zhonghua 30 and Mars were japonica like, and their TDj values were less than those of Akihikali and Zhongzuo 93 which were typical japonica. The TDj value detected by DNA was consistent with the TDj value detected by isozyme, however, DNA TDj value improved the sensitivity of indica japonica differentiation of varieties of rice.更多还原