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氧化亚铁硫杆菌生长迟缓期的影响因素

INFLUENCING FACTORS OF LAG PHASE IN GROWTH OF THIOBACILLUS FERROOXIDANS

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【作者】 张传福闵小波柴立元钟海云

【Author】 Zhang Chuanfu Min Xiaobo Chai Liyuan Zhong Haiyun (Department of Nonferrous Metallurgy, Central South University of Technology, Changsha, 410083, China)

【机构】 中南工业大学有色冶金系!长沙410083

【摘要】 以溶液中Fe2+ 的氧化表征氧化亚铁硫杆菌的生长规律,研究了初始pH 值、温度、静置与振荡培养、接种量、接种状态以及雄黄、雌黄对细菌生长迟缓期长短的影响.研究结果表明:适宜的初始pH 值(2.0~2 .5) 、温度(30~35 ℃)、振荡培养、大的接种量以及处于对数生长期的菌种有相对较短的迟缓期;雄黄、雌黄的存在,延长了细菌生长的迟缓期,抑制了细菌的生长与繁殖.

【Abstract】 The influences of some factors, such as initial pH, temperature, steady state and shake culture, inoculation amount, inoculum state, and the existence of realgar and orpiment were examined on lag phase in growth of thiobacillus ferrooxidans, with the bacterial growth being expressed by the oxidation of ferrous iron in culture solution. It was indicated that lag phase is comparatively short under the condition of pH 2.0~2.5, temperature 30~35 ℃, shaking culture, great inoculation amount and inoculum in logarithmic phase. The existence of realgar and orpiment is harmful to the growth and prolongs the lag phase.

【关键词】 氧化亚铁硫杆菌迟缓期雄黄雌黄
【Key words】 thiobacillus ferrooxidanslag phaserealgarorpiment
【基金】 国家教育部博士点基金
  • 【文献出处】 中南工业大学学报(自然科学版) ,JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE) , 编辑部邮箱 ,1999年05期
  • 【分类号】TF111.311
  • 【被引频次】61
  • 【下载频次】443
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