【摘要】 目的：探讨人多发性骨髓瘤细胞（ＭＭ）ＸＧ７选择性激发扩增人外周血γδＴ细胞机制。方法：运用蛋白质分离纯化手段获取ＭＭ细胞ＸＧ７膜蛋白组分，检查每一组分对γδＴ细胞的刺激效应，对其中的有效组分作Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ等鉴定分析。结果：证实了其中一种为热休克蛋白７０的膜蛋白能选择性扩增人外周血的γδＴ细胞。结论：表达于人多发性骨髓瘤细胞ＸＧ７细胞膜上的热休克蛋白７０多肽是γδＴ细胞所识别的配体之一。更多还原

【Abstract】 Objective:To elucidate the mechanism of γδT cell’s response to human multiple myloma(MM) cell line XG 7,and to develop a novel expansion system of γδT cell by using PBMCs stimulated with HSP70 peptide derived from XG 7.Methods:The protein isolation and purification techniques were introduced.Results:A protein which specifically stimulated γδT cells were extracted and purificated.Furthermore,this effective protein was characterized as the HSP70 molecule by western blotting.Based on the findings,successfully developed a novel culture system that selectively expanded γδT cells by using PBMCs stimulated with HPS70 peptides.Conclusion:The HSP70 peptide on XG 7cells may be one of the ligand for γδT cells.更多还原